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> Nilsson Mats
> Kalaboukhov Alexei 1975 >
Characterization of...
Characterization of Binding of Magnetic Nanoparticles to Rolling Circle Amplification Products by Turn-On Magnetic Assay
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- Sepehri, Sobhan, 1986 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden,Chalmers University of Technology, Sweden
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- Agnarsson, Björn, 1977 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Phys, Gothenburg, Sweden,Chalmers University of Technology, Sweden
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- Zardán Gómez de la Torre, Teresa (author)
- Uppsala universitet,Nanoteknologi och funktionella material,Uppsala University, Sweden
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- Schneiderman, Justin F., 1979 (author)
- Gothenburg University,Göteborgs universitet,Institutionen för neurovetenskap och fysiologi,Institute of Neuroscience and Physiology,Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden; Univ Gothenburg, MedTech West, Gothenburg, Sweden; Univ Gothenburg, Inst Neurosci & Physiol, Gothenburg, Sweden,Chalmers University of Technology, Sweden; University of Gothenburg, Sweden
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- Blomgren, Jakob (author)
- RISE,Acreo
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- Jesorka, Aldo, 1967 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers University of Technology, Sweden
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- Johansson, Christer, 1964 (author)
- RISE,Acreo
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- Nilsson, Mats (author)
- Stockholms universitet,Institutionen för biokemi och biofysik,Science for Life Laboratory (SciLifeLab),Stockholm Univ, Sci Life Lab, Dept Biochem & Biophys, Solna, Sweden,Stockholm University, Sweden
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- Albert, J. (author)
- Karolinska Institutet,Karolinska Univ Hosp, Dept Clin Microbiol, Stockholm, Sweden; Karolinska Inst, Dept Microbiol Tumor & Cell Biol, Stockholm, Sweden,Karolinska University Hospital, Sweden; Karolinska Institute, Sweden
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- Strömme, Maria, 1970- (author)
- Uppsala universitet,Nanoteknologi och funktionella material,Uppsala University, Sweden
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- Winkler, Dag, 1957 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden,Chalmers University of Technology, Sweden
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- Kalaboukhov, Alexei, 1975 (author)
- Chalmers tekniska högskola,Chalmers University of Technology,Chalmers Univ Technol, Dept Microtechnol & Nanosci MC2, Gothenburg, Sweden,Chalmers University of Technology, Sweden
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Schneiderman, Justin E. (author)
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(creator_code:org_t)
- 2019-09-17
- 2019
- English.
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In: Biosensors-Basel. - : MDPI AG. ; 9:3
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Abstract
Subject headings
Close
- The specific binding of oligonucleotide-tagged 100 nm magnetic nanoparticles (MNPs) to rolling circle products (RCPs) is investigated using our newly developed differential homogenous magnetic assay (DHMA). The DHMA measures ac magnetic susceptibility from a test and a control samples simultaneously and eliminates magnetic background signal. Therefore, the DHMA can reveal details of binding kinetics of magnetic nanoparticles at very low concentrations of RCPs. From the analysis of the imaginary part of the DHMA signal, we find that smaller MNPs in the particle ensemble bind first to the RCPs. When the RCP concentration increases, we observe the formation of agglomerates, which leads to lower number of MNPs per RCP at higher concentrations of RCPs. The results thus indicate that a full frequency range of ac susceptibility observation is necessary to detect low concentrations of target RCPs and a long amplification time is not required as it does not significantly increase the number of MNPs per RCP. The findings are critical for understanding the underlying microscopic binding process for improving the assay performance. They furthermore suggest DHMA is a powerful technique for dynamically characterizing the binding interactions between MNPs and biomolecules in fluid volumes.
Subject headings
- NATURVETENSKAP -- Fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences (hsv//eng)
- NATURVETENSKAP -- Biologi -- Biokemi och molekylärbiologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences -- Biochemistry and Molecular Biology (hsv//eng)
- NATURVETENSKAP -- Kemi -- Materialkemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences -- Materials Chemistry (hsv//eng)
- NATURVETENSKAP -- Fysik -- Den kondenserade materiens fysik (hsv//swe)
- NATURAL SCIENCES -- Physical Sciences -- Condensed Matter Physics (hsv//eng)
- NATURVETENSKAP -- Biologi (hsv//swe)
- NATURAL SCIENCES -- Biological Sciences (hsv//eng)
- NATURVETENSKAP -- Kemi (hsv//swe)
- NATURAL SCIENCES -- Chemical Sciences (hsv//eng)
- TEKNIK OCH TEKNOLOGIER -- Nanoteknik (hsv//swe)
- ENGINEERING AND TECHNOLOGY -- Nano-technology (hsv//eng)
- MEDICIN OCH HÄLSOVETENSKAP -- Medicinska och farmaceutiska grundvetenskaper -- Farmakologi och toxikologi (hsv//swe)
- MEDICAL AND HEALTH SCIENCES -- Basic Medicine -- Pharmacology and Toxicology (hsv//eng)
Keyword
- magnetic nanoparticle
- bioassay
- differential homogenous magnetic assay
- immobilization
- binding
- signal amplification
- padlock probes
- bacterial-DNA
- nanobeads
- Chemistry
- biomolecular dynamics
- Teknisk fysik med inriktning mot nanoteknologi och funktionella material
Publication and Content Type
- ref (subject category)
- art (subject category)
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- By the author/editor
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Sepehri, Sobhan, ...
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Agnarsson, Björn ...
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Zardán Gómez de ...
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Schneiderman, Ju ...
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Blomgren, Jakob
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Jesorka, Aldo, 1 ...
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Johansson, Chris ...
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Nilsson, Mats
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Albert, J.
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Strömme, Maria, ...
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Winkler, Dag, 19 ...
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Kalaboukhov, Ale ...
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Schneiderman, Ju ...
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- About the subject
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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and Biochemistry and ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Chemical Science ...
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and Materials Chemis ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Physical Science ...
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and Condensed Matter ...
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- NATURAL SCIENCES
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NATURAL SCIENCES
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and Biological Scien ...
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- Articles in the publication
- Biosensors-Basel
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Biosensors
- By the university
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University of Gothenburg
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Chalmers University of Technology
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Stockholm University
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Karolinska Institutet
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Uppsala University
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RISE